The Stem Cell Facility, located in 336 Barker Hall, supports research by providing human pluripotent stem cells, media, supplies and expertise on a recharge basis for more than 40 different laboratories on and off the main campus. The facility provides cell cultures for researchers to take to their labs for experiments, provides media and materials for checkout, and also allows use of laminar flow hoods, incubators and microscopes for researchers who are using cells provided by the Facility, to use on site. See our services section for more details below.
Services:
Expert consultation for researchers regarding cell culture needs, media, and experimental design
Grow and culture of the Allen Institute genome-engineered human iPSC cell collection, WTC-11, and others
Cell Authentication by STR analysis provided in conjunction with the DNA Sequencing Facility
Detect Karyotypic Abnormalities in Human ES and IPS cells provided in conjunction with the DNA
Sequencing Facility
Mycoplasma screening
User training in cell culture technique
Cryopreservation of cell lines
Provide tested media, serum, and cell lines
Provide and stock cell culture reagents including media, serum, additives, and other reagents
Use of 2 laminar flow hoods for mammalian or insect cell manipulation*
Contract work for outside companies
*Major equipment includes five biosafety cabinets; two phase contrast microscopes, one fluorescence microscope seven CO2 incubators, one mixed gas incubator and two incubators for insect cell lines; two incubators for large scale growth of cells using roller bottles or spinner flasks; cold storage facilities consisting of cryogenic storage dewars, one ultra low freezer, four -15° C freezers, two refrigerators and a cold room for storage. The core is located in three rooms in Barker Hall with easy access for most researchers in UCB.
Core Facilities Director: Alison Killilea
